Ultrafiltration tekinoroji ndiyo nyowani uye yakakwirira-inoshanda yekuparadzanisa tekinoroji.Iine hunhu hwemaitiro akareruka, hupfumi hwepamusoro hunobatsira, hapana shanduko yechikamu, yakakura yekuparadzanisa coefficient, kuchengetedza simba, kushanda kwakanyanya, hakuna kusvibiswa kwechipiri, kuenderera mberi kwekushanda pane tembiricha yekamuri uye zvichingodaro.Nhasi, maneja Yang anobva kuBeijing akabvunza nezve yedu ultrafiltration michina yekuchenesa mapuroteni uye akataura zvakadzama nehunyanzvi hwedu.Ikozvino, mupepeti weboka reShandong Bona achasuma mashandisirwo eiyo ultrafiltration mukuparadzanisa mapuroteni uye kucheneswa.
1. Yeprotein desalination, dealcoholization uye concentration
Izvo zvakanyanya kukosha zvekushandisa kwe ultrafiltration mukucheneswa kweprotein ndeye desalting uye kuisa pfungwa.Ultrafiltration nzira yekubvisa salination uye kutarisisa inoratidzirwa nehombe batch vhoriyamu, ipfupi yekushanda nguva uye yakanyanya kushanda kweprotein kupora.Nzira yechinyakare yesteric exclusion chromatography yekubvisa zvinhu zvakasiyana kubva kumapuroteni yakatsiviwa neazvino ultrafiltration tekinoroji, yave tekinoroji huru yeprotein desalination, dealcoholization uye concentration nhasi.Mumakore achangopfuura, tekinoroji yeultrafiltration yakashandiswa zvakanyanya mukubvisa salination uye kudzoreredza kwepamusoro kukosha kweprotein mu cheese whey uye soybean whey.Lactose uye munyu uye zvimwe zvikamu muprotein, pamwe chete nezvinodiwa chaizvo zvekubudirira kupedza desalting, de-alcoholization uye kuungana kweprotein.Iko kushandiswa kweiyo ultrafiltration tekinoroji inogona zvakare kutarisisa serospecies immunoglobulins kuti isangane nekudiwa chaiko kwegoho reprotein.
2. Kupatsanura mapuroteni
Kupatsanurwa kweprotein kunoreva nzira yekuparadzanisa chikamu cheprotein chimwe nechimwe nechikamu zvichienderana nemusiyano wezvinhu zvemuviri nekemikari (senge huremu hwemamorekuru, isoelectric point, hydrophobicity, zvichingodaro) yechikamu chepuroteni chega chega mumvura yekudyisa.Gel chromatography ndiyo imwe yenzira dzinonyanya kushandiswa pakukamuranisa biological macromolecules (kunyanya mapuroteni).Kuenzaniswa neyechinyakare chromatography, tekinoroji yekuparadzanisa tekinoroji ine tarisiro yakanaka yekushandisa muchikamu uye kugadzirwa kwemaindasitiri emapuroteni nema enzymes ane kukosha kwakakosha kwehupfumi nekuda kwekudhura kwayo uye kukwidziridzwa kuri nyore.Zai jena ndiyo yakachipa mbishi zvinhu kuwana lysozyme uye ovalbumin.Munguva pfupi yapfuura, ultrafiltration inowanzoshandiswa kuparadzanisa ovalbumin uye lysozyme kubva kune zai jena.
3. Kubviswa kweEndotoxin
Kubviswa kweEndotoxin ndeimwe yemhando huru dzekushandisa yeultrafiltration tekinoroji mukucheneswa kweprotein.Nzira yekugadzira endotoxin yakaoma zvikuru.Mukuita kwekushandisa kunoshanda, nekuti puroteni yekurapa inogadzirwa neprokaryotic kutaura sisitimu iri nyore kusanganisa ne endotoxin inogadzirwa nebhakitiriya cell wall breaking, uye endotoxin, inozivikanwawo se pyrogen, imhando ye lipopolysaccharide.Mushure mekupinda mumuviri wemunhu, zvinogona kukonzera fivha, microcirculation disturbance, endotoxic shock nezvimwe zviratidzo.Kuti uchengetedze hutano hwevanhu, zvinodikanwa kushandisa ultrafiltration tekinoroji zvakakwana kubvisa endotoxins.
Kunyangwe tekinoroji yeultrafiltration ichishandiswa zvakanyanya mukuparadzanisa uye kucheneswa kwemapuroteni, inewo zvimwe zvinogumira.Kana iyo molecular uremu hwezvigadzirwa zviviri zvichaparadzaniswa zvisingasviki ka5, hazvigone kuparadzaniswa ne ultrafiltration.Kana huremu hwechigadzirwa huri pasi pe3kD, hahukwanisi kuiswa neultrafiltration, nokuti ultrafiltration inowanzoitwa pahuwandu hwehuremu hwemembrane pa1000 NWML.
Nekuenderera mberi kwekusimudzira kweindasitiri yebioengineering, zvinodiwa zvepamusoro zvinoiswa kumberi kwekuparadzanisa kwepasi uye tekinoroji yekuchenesa.Nzira dzechinyakare dzevacuum concentration, solvent extraction, dialysis, centrifugation, precipitation uye pyrogen kubviswa hazviwanikwe kuzadzisa zvinodiwa zvekugadzira futi.Ultrafiltration tekinoroji inosungirwa kuve yakawanda uye yakawanda yakashandiswa zvakanyanya nekuti yakanakira yayo mukuparadzaniswa kweprotein.
Nguva yekutumira: Kubvumbi-20-2022